MAF D06FE-308
Samples requested
Feline
sarcomas (including both vaccine/injection- site associated (ISA) and non-ISA
sarcomas)
Study outline
We
are studying the cytogenetics of feline sarcomas, aiming to enhance existing
diagnostic tools, and working towards a better understanding of the underlying
cause of the disease.
We
have particular interest in comparing the patterns of chromosomal abnormalities
in:
a)
sarcomas associated with injection-sites
b)
sarcomas originating spontaneously with no evidence for injection association
e.g.
i) in cats that have never
received vaccinations or other injections, or
ii) primary tumors arising at sites
distant from injection sites, such as distal limb, head
Sample submission
We
would be interested to receive samples from prospective cases to include into
our study.
We
recommend that sample submission is dealt with by the referring veterinarian.
Please call or email
in advance to advise of your intent to submit samples
Sample collection and
shipping
For
each case we require the following, collected as aseptically
as possible.
-
representative, non-necrotic pieces of the tumor (preferably at least 1cm3
or equivalent) in each of the following:
1) formalin
(in a leakproof container)
2) an empty, sterile
container (refrigerate until ready to ship)
3) RNA stabilization solution (OPTIONAL- supplied upon request at least 48 hours before biopsy)
- 5-10ml EDTA blood
-
basic patient information and clinical history relating to this tumor
- any associated pathology reports
- completed owner's consent form (to be supplied on request)
Samples
should be packed securely and ship by overnight courier to the address below.
Please
include ice packs (ensuring that none of the ice packs are in direct contact
with any of the samples- we recommend wrapping each tube in bubble-wrap or
similar first).
Contact details
Rachael Thomas PhD
Room 330A, CVM Research Building
Department of Molecular Biomedical Sciences
College of Veterinary Medicine
North Carolina State University
4700 Hillsborough Street
Raleigh, NC 27606 USA
Ph: 919-513-0922
Fax: 919-513-7301
email: rachael_thomas@ncsu.edu
